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opg primary and secondary antibodies  (Servicebio Inc)

 
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    Servicebio Inc opg primary and secondary antibodies
    The effect of Shengu granules on intestinal barrier, FOXP3 cells <t>and</t> <t>OPG/RANKL</t> Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.
    Opg Primary And Secondary Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opg+primary+and+secondary+antibodies/pmc10959285-95-36-37?v=Servicebio+Inc
    Average 90 stars, based on 1 article reviews
    opg primary and secondary antibodies - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis"

    Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1320500

    The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.
    Figure Legend Snippet: The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

    Techniques Used: Expressing, Immunofluorescence



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    opg primary and secondary antibodies  (Servicebio Inc)
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    Servicebio Inc opg primary and secondary antibodies
    The effect of Shengu granules on intestinal barrier, FOXP3 cells <t>and</t> <t>OPG/RANKL</t> Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.
    Opg Primary And Secondary Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opg+primary+and+secondary+antibodies/pmc10959285-95-36-37?v=Servicebio+Inc
    Average 90 stars, based on 1 article reviews
    opg primary and secondary antibodies - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    opg and β-actin primary and secondary antibodies  (Santa Cruz Biotechnology)
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    Osteoprotegerin <t>(OPG)</t> expression: (A) Secreted OPG protein was measured by enzyme-linked immunosorbent <t>assay</t> <t>(ELISA).</t> Data were the means of triplicates indicated by standard deviation (t-test; *P < .05). (B) Western blot for OPG protein in cytoplasm. OPG protein was confirmed to overexpress in MC3T3-E1.
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    Image Search Results


    The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

    Journal: Frontiers in Microbiology

    Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis

    doi: 10.3389/fmicb.2024.1320500

    Figure Lengend Snippet: The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

    Article Snippet: Total protein samples extracted from colon tissue were incubated with ZO-1 (Servicebio Technology Ltd., China) and Occludin (Servicebio Technology Ltd., China) primary and secondary antibodies, and total protein samples extracted from femur tissue were incubated with OPG (Servicebio Technology Ltd., China) and RANKL (Servicebio Technology Ltd., China) primary and secondary antibodies.

    Techniques: Expressing, Immunofluorescence

    Osteoprotegerin (OPG) expression: (A) Secreted OPG protein was measured by enzyme-linked immunosorbent assay (ELISA). Data were the means of triplicates indicated by standard deviation (t-test; *P < .05). (B) Western blot for OPG protein in cytoplasm. OPG protein was confirmed to overexpress in MC3T3-E1.

    Journal: The Angle Orthodontist

    Article Title: Overexpression of osteoprotegerin promotes preosteoblast differentiation to mature osteoblasts

    doi: 10.2319/050210-238.1

    Figure Lengend Snippet: Osteoprotegerin (OPG) expression: (A) Secreted OPG protein was measured by enzyme-linked immunosorbent assay (ELISA). Data were the means of triplicates indicated by standard deviation (t-test; *P < .05). (B) Western blot for OPG protein in cytoplasm. OPG protein was confirmed to overexpress in MC3T3-E1.

    Article Snippet: Digestion enzyme, T4 DNA liganase, and pfu DNA polymerase from fermentas were used (St Leon-Rot, Germany); in addition, one-shot top 10 chemically competent Escherichia coli cells from Invitrogen (Breda, The Netherlands), plasmids and reverse transcriptase polymerase chain reaction (PCR) products, purification kits from Qiagen (Valencia, Calif); FuGENE 6 transfection reagents from Roche (Indianapolis, Ind), a real-time quantitative PCR one-step kit from Applied Biosystems (Foster City, Calif), an OPG enzyme-linked immunosorbent assay (ELISA) kit from R&D Systems (R&D Systems, Minneapolis, Minn), an ALP ELISA kit from ANASpec (San Jose, Calif), and OPG and β-actin primary and secondary antibodies from Santa Cruz (Heidelberg, Germany) were used.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Standard Deviation, Western Blot

    Alkaline phosphate (ALP) expression in osteoprotegerin (OPG)-expressing cells (pOPG) and the control group (pEGFP). Cells were collected and lysed to subject enzyme-linked immunosorbent assay (ELISA) measurement. Data were the means of triplicates with the standard deviation bar. ALP activity in the OPG-expressing cells (pOPG) was significantly higher than in the control group (pEGFP) (t-test; **P < .01).

    Journal: The Angle Orthodontist

    Article Title: Overexpression of osteoprotegerin promotes preosteoblast differentiation to mature osteoblasts

    doi: 10.2319/050210-238.1

    Figure Lengend Snippet: Alkaline phosphate (ALP) expression in osteoprotegerin (OPG)-expressing cells (pOPG) and the control group (pEGFP). Cells were collected and lysed to subject enzyme-linked immunosorbent assay (ELISA) measurement. Data were the means of triplicates with the standard deviation bar. ALP activity in the OPG-expressing cells (pOPG) was significantly higher than in the control group (pEGFP) (t-test; **P < .01).

    Article Snippet: Digestion enzyme, T4 DNA liganase, and pfu DNA polymerase from fermentas were used (St Leon-Rot, Germany); in addition, one-shot top 10 chemically competent Escherichia coli cells from Invitrogen (Breda, The Netherlands), plasmids and reverse transcriptase polymerase chain reaction (PCR) products, purification kits from Qiagen (Valencia, Calif); FuGENE 6 transfection reagents from Roche (Indianapolis, Ind), a real-time quantitative PCR one-step kit from Applied Biosystems (Foster City, Calif), an OPG enzyme-linked immunosorbent assay (ELISA) kit from R&D Systems (R&D Systems, Minneapolis, Minn), an ALP ELISA kit from ANASpec (San Jose, Calif), and OPG and β-actin primary and secondary antibodies from Santa Cruz (Heidelberg, Germany) were used.

    Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation, Activity Assay